Dataset Information

Amplified EPOR/JAK2 Genes Define a Unique Subtype of Acute Erythroid Leukemia.

ABSTRACT: Acute erythroid leukemia (AEL) is a unique subtype of acute myeloid leukemia characterized by prominent erythroid proliferation whose molecular basis is poorly understood. To elucidate the underlying mechanism of erythroid proliferation, we analyzed 121 AEL using whole-genome, whole-exome, and/or targeted-capture sequencing, together with transcriptome analysis of 21 AEL samples. Combining publicly available sequencing data, we found a high frequency of gains and amplifications involving EPOR/JAK2 in TP53-mutated cases, particularly those having >80% erythroblasts designated as pure erythroid leukemia (10/13). These cases were frequently accompanied by gains and amplifications of ERG/ETS2 and associated with a very poor prognosis, even compared with other TP53-mutated AEL. In addition to activation of the STAT5 pathway, a common feature across all AEL cases, these AEL cases exhibited enhanced cell proliferation and heme metabolism and often showed high sensitivity to ruxolitinib in vitro and in xenograft models, highlighting a potential role of JAK2 inhibition in therapeutics of AEL.

Significance

This study reveals the major role of gains, amplifications, and mutations of EPOR and JAK2 in the pathogenesis of pure erythroleukemia. Their frequent response to ruxolitinib in patient-derived xenograft and cell culture models highlights a possible therapeutic role of JAK2 inhibition for erythroleukemia with EPOR/JAK2-involving lesions. This article is highlighted in the In This Issue feature, p. 369.

SUBMITTER: Takeda J

PROVIDER: S-EPMC9894574 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Amplified EPOR/JAK2 Genes Define a Unique Subtype of Acute Erythroid Leukemia.

Takeda June J Yoshida Kenichi K Nakagawa Masahiro M MM Nannya Yasuhito Y Yoda Akinori A Saiki Ryunosuke R Ochi Yotaro Y Zhao Lanying L Okuda Rurika R Qi Xingxing X Mori Takuto T Kon Ayana A Chiba Kenichi K Tanaka Hiroko H Shiraishi Yuichi Y Kuo Ming-Chung MC Kerr Cassandra M CM Nagata Yasunobu Y Morishita Daisuke D Hiramoto Nobuhiro N Hangaishi Akira A Nakazawa Hideyuki H Ishiyama Ken K Miyano Satoru S Chiba Shigeru S Miyazaki Yasushi Y Kitano Toshiyuki T Usuki Kensuke K Sezaki Nobuo N Tsurumi Hisashi H Miyawaki Shuichi S Maciejewski Jaroslaw P JP Ishikawa Takayuki T Ohyashiki Kazuma K Ganser Arnold A Heuser Michael M Thol Felicitas F Shih Lee-Yung LY Takaori-Kondo Akifumi A Makishima Hideki H Ogawa Seishi S

Blood cancer discovery 20220901 5

Acute erythroid leukemia (AEL) is a unique subtype of acute myeloid leukemia characterized by prominent erythroid proliferation whose molecular basis is poorly understood. To elucidate the underlying mechanism of erythroid proliferation, we analyzed 121 AEL using whole-genome, whole-exome, and/or targeted-capture sequencing, together with transcriptome analysis of 21 AEL samples. Combining publicly available sequencing data, we found a high frequency of gains and amplifications involving EPOR/JA ...[more]

PMID: 35839275

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Dataset Information

Amplified EPOR/JAK2 Genes Define a Unique Subtype of Acute Erythroid Leukemia.

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Publications

Amplified EPOR/JAK2 Genes Define a Unique Subtype of Acute Erythroid Leukemia.

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