Alkaline sphingomyelinase (NPP7) impacts the homeostasis of intestinal T lymphocyte populations.

ABSTRACT:

Background and aim

Alkaline sphingomyelinase (NPP7) is expressed by intestinal epithelial cells and is crucial for the digestion of dietary sphingomyelin. NPP7 also inactivates proinflammatory mediators including platelet-activating factor and lysophosphatidylcholine. The aim of this study was to examine a potential role for NPP7 in the homeostasis of the intestinal immune system.

Methods

We quantified the numbers of B-lymphocytes, plasma cells, T-lymphocytes including regulatory T-lymphocytes (T_regs), natural killer cells, dendritic cells, macrophages, and neutrophils, in the small and large intestines, the mesenteric lymph nodes and the spleens of heterozygous and homozygous NPP7 knockout (KO) and wildtype (WT) mice. Tissues were examined by immunohistochemistry

SUBMITTER: Alyamani M 

PROVIDER: S-EPMC9894718 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature