Single-cell analysis reveals the COL11A1⁺ fibroblasts are cancer-specific fibroblasts that promote tumor progression.

ABSTRACT: Background: Cancer-associated fibroblasts (CAFs) promote tumor progression through extracellular matrix (ECM) remodeling and extensive communication with other cells in tumor microenvironment. However, most CAF-targeting strategies failed in clinical trials due to the heterogeneity of CAFs. Hence, we aimed to identify the cluster of tumor-promoting CAFs, elucidate their function and determine their specific membrane markers to ensure precise targeting. Methods: We integrated multiple single-cell RNA sequencing (scRNA-seq) datasets across different tumors and adjacent normal tissues to identify the tumor-promoting CAF cluster. We analyzed the origin of these CAFs by pseudotime analysis, and tried to elucidate the function of these CAFs by gene regulatory network analysis and c

SUBMITTER: Zhang J 

PROVIDER: S-EPMC9894880 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature