PLATERO: A calibration protocol for plate reader green fluorescence measurements.

ABSTRACT: One of the most common sources of information in Synthetic Biology is the data coming from plate reader fluorescence measurements. These experiments provide a measure of the light emitted by a certain fluorescent molecule, such as the Green Fluorescent Protein (GFP). However, these measurements are generally expressed in arbitrary units and are affected by the measurement device gain. This limits the range of measurements in a single experiment and hampers the comparison of results among experiments. In this work, we describe PLATERO, a calibration protocol to express fluorescence measures in concentration units of a reference fluorophore. The protocol removes the gain effect of the measurement device on the acquired data. In addition, the fluorescence intensity values are transformed into

SUBMITTER: Gonzalez-Cebrian A 

PROVIDER: S-EPMC9895789 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature