Blood tissue Plasminogen Activator (tPA) of liver origin contributes to neurovascular coupling involving brain endothelial N-Methyl-D-Aspartate (NMDA) receptors.

ABSTRACT:

Background

Regulation of cerebral blood flow (CBF) directly influence brain functions and dysfunctions and involves complex mechanisms, including neurovascular coupling (NVC). It was suggested that the serine protease tissue-type plasminogen activator (tPA) could control CNV induced by whisker stimulation in rodents, through its action on N-methyl-D-Aspartate receptors (NMDARs). However, the origin of tPA and the location and mechanism of its action on NMDARs in relation to CNV remained debated.

Methods

Here, we answered these issues using tPA^Null mice, conditional deletions of either endothelial tPA (VECad-Cre^ΔtPA) or endothelial GluN1 subunit of NMDARs (VECad-Cre^ΔGluN1), parabioses between wild-type and tPA^Null mice, hydrodynamic

SUBMITTER: Furon J 

PROVIDER: S-EPMC9896721 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature