5-HT attenuates chronic stress-induced cognitive impairment in mice through intestinal flora disruption.

ABSTRACT:

Background

The microbiota-gut-brain axis plays an important role in the development of depression. The aim of this study was to investigate the effects of 5-HT on cognitive function, learning and memory induced by chronic unforeseeable mild stress stimulation (CUMS) in female mice. CUMS mice and TPH2 KO mice were used in the study. Lactococcus lactis E001-B-8 fungus powder was orally administered to mice with CUMS.

Methods

We used the open field test, Morris water maze, tail suspension test and sucrose preference test to examine learning-related behaviours. In addition, AB-PAS staining, immunofluorescence, ELISA, qPCR, Western blotting and microbial sequencing were employed to address our hypotheses.

Results

The effect of CUMS was more obvious in female mice than in m

SUBMITTER: Ma J 

PROVIDER: S-EPMC9896737 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature