ShrinkCRISPR: a flexible method for differential fitness analysis of CRISPR-Cas9 screen data.

ABSTRACT:

Background

CRISPR screens provide large-scale assessment of cellular gene functions. Pooled libraries typically consist of several single guide RNAs (sgRNAs) per gene, for a large number of genes, which are transduced in such a way that every cell receives at most one sgRNA, resulting in the disruption of a single gene in that cell. This approach is often used to investigate effects on cellular fitness, by measuring sgRNA abundance at different time points. Comparing gene knockout effects between different cell populations is challenging due to variable cell-type specific parameters and between replicates variation. Failure to take those into account can lead to inflated or false discoveries.

Results

We propose a new, flexible approach called ShrinkCRISPR that can take into

SUBMITTER: Tissier RLM 

PROVIDER: S-EPMC9896759 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature