In vitro culture of the zoonotic nematode Anisakis pegreffii (Nematoda, Anisakidae).

ABSTRACT:

Background

Anisakiasis is a foodborne disease caused by the third-stage larvae (L3) of two species belonging to the genus Anisakis: Anisakis pegreffii and Anisakis simplex sensu stricto. Both species have been the subject of different -omics studies undertaken in the past decade, but a reliable in vitro culture protocol that would enable a more versatile approach to functional studies has never been devised. In nature, A. pegreffii shows a polyxenous life-cycle. It reproduces in toothed whales (final host) and disseminates embryonated eggs via cetacean faeces in the water column. In the environment, a first- (L1) and second-stage larva (L2) develops inside the egg, and subsequently hatched L2 is ingested by a planktonic crustacean or small fish (intermediate host). In the crustacea

SUBMITTER: Mladineo I 

PROVIDER: S-EPMC9896804 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature