Increased IL-1α expression is correlated with bladder cancer malignant progression.

ABSTRACT:

Introduction

To explore the function of interleukin 1α (IL-1α) in bladder cancer (BCa).

Material and methods

Immunohistochemistry (IHC) was used to test the protein expression of IL-1α in BCa tissues. The relationship between IL-1α and clinical characteristics was analyzed by the Kaplan-Meier curve method. The gene and protein expression was tested by reverse transcription quantitative polymerase chain reaction (RT-q-PCR) and western blot, respectively. Colony formation and MTT assays were used to detect the potential of proliferation in vitro, and scratch and transwell chamber assays were used to detect the potential of invasion in vitro. Markers of proliferation such as Ki-67 and proliferating cell nuclear antigen (PCNA) and markers of invasion such as

SUBMITTER: Yao SJ 

PROVIDER: S-EPMC9897080 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature