A Mass Spectrometry-Based Method of Quantifying the Contribution of the Lysine Polymorphism at Position 171 in Sheep PrP.

ABSTRACT: In sheep, the transmissibility and progression of scrapie, a sheep prion (PrP^Sc) disease, is strongly dependent upon specific amino acid polymorphisms in the natively expressed prion protein (PrP^C). Sheep expressing PrP^C with lysine (K) polymorphism at position 171 (K171) are partially resistant to oronasal dosing of classical sheep scrapie. In addition, scrapie infected sheep expressing the K171 polymorphism show a longer incubation period compared to sheep homozygous (glutamine (Q)) at position 171. Quantitating the amount of the K171 polymorphism in a sheep scrapie sample can provide important information on the composition of PrP^Sc. A tryptic peptide, ₁₅₉R.YPNQVYYRPVDK.Y₁₇₂, derived from the digestion of 171K recombinant

SUBMITTER: Silva CJ 

PROVIDER: S-EPMC9897214 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature