Dysregulation of CD177⁺ neutrophils on intraepithelial lymphocytes exacerbates gut inflammation via decreasing microbiota-derived DMF.

ABSTRACT: Neutrophils synergize with intestinal resident intraepithelial lymphocytes (IELs) to serve as the first-line defense and maintain intestinal homeostasis. However, the underlying mechanisms whereby neutrophils regulate IELs to inhibit intestinal inflammation are still not completely understood. Here, we found that depletion of neutrophils (especially CD177⁺ subset) caused expansion of colitogenic TCRγδ⁺CD8αα⁺ IELs, increased intestinal inflammation, and dysbiosis after dextran sulfate sodium exposure or Citrobacter rodentium infection in mice. scRNA-seq analysis revealed a pyroptosis-related gene signature and hyperresponsiveness to microbiota in TCRγδ⁺CD8αα⁺ IELs from colitic Cd177^-/- mice. Microbiota-de

SUBMITTER: Chen H 

PROVIDER: S-EPMC9897772 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature