Estradiol deficiency and skeletal muscle apoptosis: Possible contribution of microRNAs.

ABSTRACT:

Background

Menopause leads to estradiol (E₂) deficiency that is associated with decreases in muscle mass and strength. Here we studied the effect of E₂ deficiency on microRNA (miR) signaling that targets apoptotic pathways.

Methods

C57BL6 mice were divided into control (normal estrous cycle, n = 8), OVX (E₂ deficiency, n = 7) and OVX + E₂ groups (E₂-pellet, n = 4). Six weeks following the OVX surgery, mice were sacrificed and RNA isolated from gastrocnemius muscles. miR-profiles were studied with Next-Generation Sequencing (NGS) and candidate miRs verified using qPCR. The target proteins of the miRs were found using in silico analysis and measured at mRNA (qPCR) and protein levels (Western blot).

Results

Of the ap

SUBMITTER: Karvinen S 

PROVIDER: S-EPMC9897888 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature