Transient regulation of RNA methylation in human hematopoietic stem cells promotes their homing and engraftment.

ABSTRACT: Enhancing the efficiency of hematopoietic stem cell (HSC) homing and engraftment is critical for cord blood (CB) hematopoietic cell transplantation (HCT). Recent studies indicate that N⁶-methyladenosine (m⁶A) modulates the expression of mRNAs that are critical for stem cell function by influencing their stability. Here, we demonstrate that inhibition of RNA decay by regulation of RNA methylation, enhances the expression of the homing receptor chemokine C-X-C receptor-4 (CXCR4) in HSCs. We show that YTH N6-methyladenosine RNA binding protein 2 (YTHDF2), a m⁶A reader and FTO α-ketoglutarate dependent dioxygenase (FTO), a m⁶A eraser play an opposite role in this process. Through screening, we identified several FDA-approved compounds that regulate t

SUBMITTER: Wang X 

PROVIDER: S-EPMC9898034 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature