Analysis and modeling of SDS and DPC micelle SAXS data for membrane protein solution structure characterization.

ABSTRACT: Herein, we present analysis and analytical modeling of Small Angle X-ray Scattering (SAXS) data on two surfactants forming micelles (i.e., sodium dodecyl sulfate and dodecyl phosphocholine) and used for the study in solution of mTSPO, the translocator membrane protein from Mus musculus, as supporting data of the research article published in Biochimie (Combet et al., 2022). For both surfactants, concentration series were measured at two Synchrotron SAXS-beamlines. After reduction, buffer subtraction and water calibration of the data, SAXS curves were normalized to surfactant concentration to highlight possible changes in micelle shape or presence of inter-micellar weak interactions. They were then analyzed in terms of radius of gyration (R _G), absolute forward inte

SUBMITTER: Pozza A 

PROVIDER: S-EPMC9898585 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature