Spatial control of self-organizing vascular networks with programmable aptamer-tethered growth factor photopatterning.

ABSTRACT: Given the dynamic nature of engineered vascular networks within biofabricated tissue analogues, it is instrumental to have control over the constantly evolving biochemical cues within synthetic matrices throughout tissue remodeling. Incorporation of pro-angiogenic vascular endothelial growth factor (VEGF₁₆₅) specific aptamers into cell-instructive polymer networks is shown to be pivotal for spatiotemporally controlling the local bioactivity of VEGF that selectively elicit specific cell responses. To harness this effect and quantitatively unravel its spatial resolution, herein, bicomponent micropatterns consisting of VEGF₁₆₅ specific aptamer-functionalized gelatin methacryloyl (GelMA) (aptamer regions) overlaid with pristine GelMA regions using visible-light photoiniti

SUBMITTER: Rana D 

PROVIDER: S-EPMC9898740 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature