Murine skin-derived multipotent papillary dermal fibroblast progenitors show germline potential in vitro.

ABSTRACT:

Background

Many laboratories have described the in vitro isolation of multipotent cells with stem cell properties from the skin of various species termed skin-derived stem cells (SDSCs). However, the cellular origin of these cells and their capability to give rise, among various cell types, to male germ cells, remain largely unexplored.

Methods

SDSCs were isolated from newborn mice skin, and then differentiated into primordial germ cell-like cells (PGCLCs) in vitro. Single-cell RNA sequencing (scRNA-seq) was then applied to dissect the cellular origin of SDSCs using cells isolated from newborn mouse skin and SDSC colonies. Based on an optimized culture strategy, we successfully generated spermatogonial stem cell-like cells (SSCLCs) in vitro.

Results

Here, using scRNA-

SUBMITTER: Ge W 

PROVIDER: S-EPMC9898921 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature