Coordinated local RNA overexpression of complement induced by interferon gamma in myositis.

ABSTRACT: Complement proteins are deposited in the muscles of patients with myositis. However, the local expression and regulation of complement genes within myositis muscle have not been well characterized. In this study, bulk RNA sequencing (RNAseq) analyses of muscle biopsy specimens revealed that complement genes are locally overexpressed and correlate with markers of myositis disease activity, including the expression of interferon-gamma (IFNγ)-induced genes. Single cell and single nuclei RNAseq analyses showed that most local expression of complement genes occurs in macrophages, fibroblasts, and satellite cells, with each cell type expressing different sets of complement genes. Biopsies from immune-mediated necrotizing myopathy patients, who have the lowest levels of IFNγ-induced genes, also h

SUBMITTER: Casal-Dominguez M 

PROVIDER: S-EPMC9899209 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature