ASC specks exacerbate α‑synuclein pathology via amplifying NLRP3 inflammasome activities.

ABSTRACT:

Background

Inflammasome activation has a pathogenic role in Parkinson's disease (PD). Up-regulated expressions of inflammasome adaptor apoptosis-associated speck-like protein containing a CARD (ASC) and assembly of ASC specks have been observed in postmortems of human PD brains and experimental PD models. Extracellular ASC specks behave like danger signals and sustain prolonged inflammasome activation. However, the contribution of ASC specks in propagation of inflammasome activation and pathological progression in PD has not been fully established.

Methods

Herein, we used human A53T mutant α-synuclein preformed fibrils (PFFs)-stimulated microglia in vitro and unilateral striatal stereotaxic injection of PFFs-induced mice model of PD in vivo, to investigate the significance o

SUBMITTER: Zheng R 

PROVIDER: S-EPMC9899382 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature