Endothelial Toll-like receptor 4 is required for microglia activation in the murine retina after systemic lipopolysaccharide exposure.

ABSTRACT:

Background

Clustering of microglia around the vasculature has been reported in the retina and the brain after systemic administration of lipopolysaccharides (LPS) in mice. LPS acts via activation of Toll-like receptor 4 (TRL4), which is expressed in several cell types including microglia, monocytes and vascular endothelial cells. The purpose of this study was to investigate the effect of systemic LPS in the pigmented mouse retina and the involvement of endothelial TLR4 in LPS-induced retinal microglia activation.

Methods

C57BL/6J, conditional knockout mice that lack Tlr4 expression selectively on endothelial cells (Tek^Cre-posTlr4^loxP/loxP) and Tek^Cre-negTlr4^loxP/loxP mice were used. The mice were injected with 1 mg/kg LPS via the t

SUBMITTER: Tsioti I 

PROVIDER: S-EPMC9899393 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature