Anammox bacterial abundance and diversity in different temperatures of purple paddy soils by ¹³C-DNA stable-isotope probing combined with high-throughput sequencing.

ABSTRACT:

Introduction

Anaerobic ammonium oxidation (anammox) plays a vital role in the global nitrogen cycle by oxidizing ammonium to nitrogen under anaerobic environments. However, the existence, abundance, and diversity of anammox bacteria between different temperatures are less studied, particularly in purple paddy soils.

Methods

¹³C-DNA stable-isotope probe combined with Illumina MiSeq high-throughput sequencing was employed to explore soil abundance and diversity of anammox bacteria. In doing so, 40-60 cm depth soils from typical purple paddy soils in Chongqing, southwest China, were cultured under ¹²CO₂-labeled and ¹³CO₂-labeled at 35°C, 25°C, 15°C, and 5°C for 56 days.

Results and discussion

Anammox bacteria were no

SUBMITTER: Yu Z 

PROVIDER: S-EPMC9899793 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature