Intracellular virion traffic to the endosome driven by cell type specific sialic acid receptors determines parvovirus tropism.

ABSTRACT: Parvoviruses are promising anticancer and gene therapy agents, but a deep knowledge of the entry process is crucial to exploit their therapeutic potential. We addressed this issue while attempting to retarget the oncolytic parvovirus minute virus of mice (MVMp) to the tumor vasculature. Residues at three functional domains of the icosahedral capsid were substituted by rational design with peptides competing with the vascular endothelial growth factor. Most substitutions impaired virus maturation, though some yielded infectious chimeric virions, and substitutions in a dimple at the twofold axis that allocates sialic acid (SIA) receptors altered viral tropism. One dimple-modified chimeric virion was efficiently attached as MVMp to α2-linked SIA moieties, but the infection was impaired by the

SUBMITTER: Calvo-Lopez T 

PROVIDER: S-EPMC9899843 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature