Macromolecular crowding and decellularization method increase the growth factor binding potential of cell-secreted extracellular matrices.

ABSTRACT: Recombinant growth factors are used in tissue engineering to stimulate cell proliferation, migration, and differentiation. Conventional methods of growth factor delivery for therapeutic applications employ large amounts of these bioactive cues. Effective, localized growth factor release is essential to reduce the required dose and potential deleterious effects. The endogenous extracellular matrix (ECM) sequesters native growth factors through its negatively charged sulfated glycosaminoglycans. Mesenchymal stromal cells secrete an instructive extracellular matrix that can be tuned by varying culture and decellularization methods. In this study, mesenchymal stromal cell-secreted extracellular matrix was modified using λ-carrageenan as a macromolecular crowding (MMC) agent and decellularized

SUBMITTER: Fok SW 

PROVIDER: S-EPMC9899907 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature