Application of nanopore adaptive sequencing in pathogen detection of a patient with Chlamydia psittaci infection.

ABSTRACT:

Introduction

Nanopore sequencing has been widely used in clinical metagenomic sequencing for pathogen detection with high portability and real-time sequencing. Oxford Nanopore Technologies has recently launched an adaptive sequencing function, which can enrich on-target reads through real-time alignment and eject uninteresting reads by reversing the voltage across the nanopore. Here we evaluated the utility of adaptive sequencing in clinical pathogen detection.

Methods

Nanopore adaptive sequencing and standard sequencing was performed on a same flow cell with a bronchoalveolar lavage fluid sample from a patient with Chlamydia psittacosis infection, and was compared with the previous mNGS results.

Results

Nanopore adaptive sequencing identified 648 on-target sto

SUBMITTER: Lin Y 

PROVIDER: S-EPMC9900021 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature