Autophagy-related IFNG is a prognostic and immunochemotherapeutic biomarker of COAD patients.

ABSTRACT:

Background

Numerous studies have shown autophagy affects cellular immune responses. This study aims to explore prognosis and immunotherapeutic biomarkers related to autophagy in colon adenocarcinoma (COAD).

Methods

Based on R software, we performed the ssGSEA, differential expression analysis, Kaplan-Meier survival analysis, correlation analysis, and enrichment analysis. For wet experiment, we did qRT-PCR, immunohistochemistry and CCK-8 experiments.

Results

Using autophagy-related genes (ARGs) and the ssGSEA, COAD patients were divided into low and high autophagy groups. For immune score, stromal score, tumor purity, tumor infiltrating immune cells, co-signaling molecules, tumor mutational burden, microsatellite instability, mismatch repair, immune-related pathways, i

SUBMITTER: Yue T 

PROVIDER: S-EPMC9900120 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature