Bi-fluorescent Staphylococcus aureus infection enables single-cell analysis of intracellular killing in vivo.

ABSTRACT: Techniques for studying the clearance of bacterial infections are critical for advances in understanding disease states, immune cell effector functions, and novel antimicrobial therapeutics. Intracellular killing of Staphylococcus aureus by neutrophils can be monitored using a S. aureus strain stably expressing GFP, a fluorophore that is quenched when exposed to the reactive oxygen species (ROS) present in the phagolysosome. Here, we expand upon this method by developing a bi-fluorescent S. aureus killing assay for use in vivo. Conjugating S. aureus with a stable secondary fluorescent marker enables the separation of infected cell samples into three populations: cells that have not engaged in phagocytosis, cells that have engulfed and killed S. aureus<

SUBMITTER: Hinman KD 

PROVIDER: S-EPMC9900177 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature