Parallelization with Dual-Trap Single-Column Configuration Maximizes Throughput of Proteomic Analysis.

ABSTRACT: Proteomic analysis on the scale that captures population and biological heterogeneity over hundreds to thousands of samples requires rapid mass spectrometry methods, which maximize instrument utilization (IU) and proteome coverage while maintaining precise and reproducible quantification. To achieve this, a short liquid chromatography gradient paired to rapid mass spectrometry data acquisition can be used to reproducibly quantify a moderate set of analytes. High-throughput profiling at a limited depth is becoming an increasingly utilized strategy for tackling large sample sets but the time spent on loading the sample, flushing the column(s), and re-equilibrating the system reduces the ratio of meaningful data acquired to total operation time and IU. The dual-trap single-column configuratio

SUBMITTER: Kreimer S 

PROVIDER: S-EPMC9900495 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature