Phosphorylation Regulation of a Histone-like HU Protein from Deinococcus radiodurans.

ABSTRACT:

Background

Histone-like proteins are small molecular weight DNA-binding proteins that are widely distributed in prokaryotes. These proteins have multiple functions in cellular structures and processes, including the morphological stability of the nucleoid, DNA compactness, DNA replication, and DNA repair. Deinococcus radiodurans, an extremophilic microorganism, has extraordinary DNA repair capability and encodes an essential histone-like protein, DrHU.

Objective

We aim to investigate the phosphorylation regulation role of a histone-like HU protein from Deinococcus radiodurans.

Methods

LC-MS/MS analysis was used to determine the phosphorylation site of endogenous DrHU. The predicted structure of DrHU-DNA was obtained from homology modeling (Swissmodel) using Staphyloco

SUBMITTER: Hou J 

PROVIDER: S-EPMC9900698 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature