Multimodal characterization of antigen-specific CD8 ⁺ T cells across SARS-CoV-2 vaccination and infection.

ABSTRACT: The human immune response to SARS-CoV-2 antigen after infection or vaccination is defined by the durable production of antibodies and T cells. Population-based monitoring typically focuses on antibody titer, but there is a need for improved characterization and quantification of T cell responses. Here, we utilize multimodal sequencing technologies to perform a longitudinal analysis of circulating human leukocytes collected before and after BNT162b2 immunization. Our data reveal distinct subpopulations of CD8 ⁺ T cells which reliably appear 28 days after prime vaccination (7 days post boost). Using a suite of cross-modality integration tools, we define their transcriptome, accessible chromatin landscape, and immunophenotype, and identify unique biomarkers within each modality. By

SUBMITTER: Zhang B 

PROVIDER: S-EPMC9900816 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature