Three-dimensional imaging and quantification of mouse ovarian follicles via optical coherence tomography.

ABSTRACT: Ovarian tissue cryopreservation has been successfully applied worldwide for fertility preservation. Correctly selecting the ovarian tissue with high follicle loading for freezing and reimplantation increases the likelihood of restoring ovarian function, but it is a challenging process. In this work, we explore the use of three-dimensional spectral-domain optical coherence tomography (SD-OCT) to identify different follicular stages, especially primary follicles, compare the identifications with H&E images, and measure the size and age-related follicular density distribution differences in mice ovaries. We use the thickness of the layers of granulosa cells to differentiate primordial and primary follicles from secondary follicles. The measured dimensions and age-related follicular distributi

SUBMITTER: Amaral MM 

PROVIDER: S-EPMC9900855 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature