Production, purification, characterization and application of two novel endoglucanases from buffalo rumen metagenome.

ABSTRACT:

Background

Lignocellulose biomass is the most abundant and renewable material in nature. The objectives of this study were to characterize two endoglucanases TrepCel3 and TrepCel4, and determine the effect of the combination of them (1.2 mg TrepCel3, 0.8 mg TrepCel4) on in vitro rumen fermentation characteristics. In this study, three nature lignocellulosic substrates (rice straw, RS; wheat straw, WS; leymus chinensis, LC) were evaluated for their in vitro digestibility, gas, NH₃-N and volatile fatty acid (VFA) production, and microbial protein (MCP) synthesis by adding enzymatic combination.

Methods

Two endoglucanases' genes were successfully expressed in Escherichia coli (E. coli) BL21 (DE3), and enzymatic characteristics were further characterized. The combinat

SUBMITTER: Meng Z 

PROVIDER: S-EPMC9900955 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature