Intraluminal chloride regulates lung branching morphogenesis: involvement of PIEZO1/PIEZO2.

ABSTRACT:

Background

Clinical and experimental evidence shows lung fluid volume as a modulator of fetal lung growth with important value in treating fetal lung hypoplasia. Thus, understanding the mechanisms underlying these morphological dynamics has been the topic of multiple investigations with, however, limited results, partially due to the difficulty of capturing or recapitulating these movements in the lab. In this sense, this study aims to establish an ex vivo model allowing the study of lung fluid function in branching morphogenesis and identify the subsequent molecular/ cellular mechanisms.

Methods

Ex vivo lung explant culture was selected as a model to study branching morphogenesis, and intraluminal injections were performed to change the composition of lung fluid. Distinct c

SUBMITTER: Goncalves AN 

PROVIDER: S-EPMC9901166 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature