FilamentSensor 2.0: An open-source modular toolbox for 2D/3D cytoskeletal filament tracking.

ABSTRACT: Cytoskeletal pattern formation and structural dynamics are key to a variety of biological functions and a detailed and quantitative analysis yields insight into finely tuned and well-balanced homeostasis and potential pathological alterations. High content life cell imaging of fluorescently labeled cytoskeletal elements under physiological conditions is nowadays state-of-the-art and can record time lapse data for detailed experimental studies. However, systematic quantification of structures and in particular the dynamics (i.e. frame-to-frame tracking) are essential. Here, an unbiased, quantitative, and robust analysis workflow that can be highly automatized is needed. For this purpose we upgraded and expanded our fiber detection algorithm FilamentSensor (FS) to the FilamentSensor 2.0 (FS2

SUBMITTER: Hauke L 

PROVIDER: S-EPMC9901806 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature