Diagnostic performance of a novel antigen-capture ELISA for the detection of SARS-CoV-2.

ABSTRACT:

Background and aims

The coronavirus disease 2019 (COVID-19) pandemic is a serious health problem worldwide. Early virus detection is essential for disease control and management. Viral antigen detection by ELISA is a cost-effective, rapid, and accurate antigen diagnostic assay which could facilitate early viral detection.

Method

An antigen-capture sandwich ELISA was developed using novel nucleocapsid (NP)-specific mouse monoclonal antibodies (MAbs). The clinical performance of the assay was assessed using 403 positive and 150 negative respiratory samples collected during different SARS-CoV-2 variants outbreaks in Iran.

Results

The limit of detection of our ELISA assay was found to be 43.3 pg/ml for recombinant NP. The overall sensitivity and specificity of this assay

SUBMITTER: Yadegari H 

PROVIDER: S-EPMC9902293 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature