Dual incorporation of non-canonical amino acids enables production of post-translationally modified selenoproteins.

ABSTRACT: Post-translational modifications (PTMs) can occur on almost all amino acids in eukaryotes as a key mechanism for regulating protein function. The ability to study the role of these modifications in various biological processes requires techniques to modify proteins site-specifically. One strategy for this is genetic code expansion (GCE) in bacteria. The low frequency of post-translational modifications in bacteria makes it a preferred host to study whether the presence of a post-translational modification influences a protein's function. Genetic code expansion employs orthogonal translation systems engineered to incorporate a modified amino acid at a designated protein position. Selenoproteins, proteins containing selenocysteine, are also known to be post-translationally modified. Selenopr

SUBMITTER: Morosky P 

PROVIDER: S-EPMC9902344 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature