25-hydroxyvitamin D₃ generates immunomodulatory plasticity in human periodontal ligament-derived mesenchymal stromal cells that is inflammatory context-dependent.

ABSTRACT:

Introduction

Human periodontal ligament-derived mesenchymal stromal cells (hPDL-MSCs) exhibit a tight bi-directional interaction with CD4⁺ T lymphocytes. The hPDL-MSCs' immunomodulatory abilities are drastically enhanced by pro-inflammatory cytokines via boosting the expression of various immunomediators. 25-hydroxyvitamin D₃ (25(OH)D₃), the major metabolite of vitamin D3 in the blood, affects both hPDL-MSCs and CD4⁺ T lymphocytes, but its influence on their interaction is unknown.

Methods

Therefore, primary hPDL-MSCs were stimulated in vitro with tumor necrosis factor (TNF)-α a or interleukin (IL)-1β in the absence and presence of 25(OH)D₃ followed by an indirect co-culture with phytohemagglutinin-activated CD4

SUBMITTER: Behm C 

PROVIDER: S-EPMC9902380 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature