Evaluation of computational phage detection tools for metagenomic datasets.

ABSTRACT:

Introduction

As new computational tools for detecting phage in metagenomes are being rapidly developed, a critical need has emerged to develop systematic benchmarks.

Methods

In this study, we surveyed 19 metagenomic phage detection tools, 9 of which could be installed and run at scale. Those 9 tools were assessed on several benchmark challenges. Fragmented reference genomes are used to assess the effects of fragment length, low viral content, phage taxonomy, robustness to eukaryotic contamination, and computational resource usage. Simulated metagenomes are used to assess the effects of sequencing and assembly quality on the tool performances. Finally, real human gut metagenomes and viromes are used to assess the differences and similarities in the phage communities predicted

SUBMITTER: Schackart KE 

PROVIDER: S-EPMC9902911 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature