Vascular inflammation on a chip: A scalable platform for trans-endothelial electrical resistance and immune cell migration.

ABSTRACT: The vasculature system plays a critical role in inflammation processes in the body. Vascular inflammatory mechanisms are characterized by disruption of blood vessel wall permeability together with increased immune cell recruitment and migration. There is a critical need to develop models that fully recapitulate changes in vascular barrier permeability in response to inflammatory conditions. We developed a scalable platform for parallel measurements of trans epithelial electrical resistance (TEER) in 64 perfused microfluidic HUVEC tubules under inflammatory conditions. Over 250 tubules where exposed to Tumor necrosis factor alpha (TNFα) and interferon gamma (INF-γ) or human peripheral blood mononuclear cells. The inflammatory response was quantified based on changes TEER and expression of I

SUBMITTER: Ehlers H 

PROVIDER: S-EPMC9903066 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature