AlacatDesigner─Computational Design of Peptide Concatamers for Protein Quantitation.

ABSTRACT: Protein quantitation via mass spectrometry relies on peptide proxies for the parent protein from which abundances are estimated. Owing to the variability in signal from individual peptides, accurate absolute quantitation usually relies on the addition of an external standard. Typically, this involves stable isotope-labeled peptides, delivered singly or as a concatenated recombinant protein. Consequently, the selection of the most appropriate surrogate peptides and the attendant design in recombinant proteins termed QconCATs are challenges for proteome science. QconCATs can now be built in a "a-la-carte" assembly method using synthetic biology: ALACATs. To assist their design, we present "AlacatDesigner", a tool that supports the peptide selection for recombinant protein standards based on

SUBMITTER: Rusilowicz M 

PROVIDER: S-EPMC9903321 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature