Single-cell-resolved dynamics of chromatin architecture delineate cell and regulatory states in zebrafish embryos.

ABSTRACT: DNA accessibility of cis-regulatory elements (CREs) dictates transcriptional activity and drives cell differentiation during development. While many genes regulating embryonic development have been identified, the underlying CRE dynamics controlling their expression remain largely uncharacterized. To address this, we produced a multimodal resource and genomic regulatory map for the zebrafish community, which integrates single-cell combinatorial indexing assay for transposase-accessible chromatin with high-throughput sequencing (sci-ATAC-seq) with bulk histone PTMs and Hi-C data to achieve a genome-wide classification of the regulatory architecture determining transcriptional activity in the 24-h post-fertilization (hpf) embryo. We characterized the genome-wide chromatin architecture

SUBMITTER: McGarvey AC 

PROVIDER: S-EPMC9903790 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature