Dataset Information

Antibiotic resistance and mecA characterization of Staphylococcus hominis from filarial lymphedema patients in the Ahanta West District, Ghana: A cross-sectional study.

ABSTRACT:

Background and aim

Filarial infections affect over 150 million people in the tropics. One of the major forms of filarial pathologies is lymphedema; a condition where the immune response is significantly altered, resulting in changes in the normal flora. Staphylococcus hominis, a human skin commensal, can also be pathogenic in immunocompromised individuals. Therefore, there is the possibility that S. hominis could assume a different behavior in filarial lymphedema patients. To this end, we investigated the levels of antibiotic resistance and extent of mecA gene carriage in S. hominis among individuals presenting with filarial lymphedema in rural Ghana.

Method

We recruited 160 individuals with stages I-VII lymphedema, in a cross-sectional study in the Ahanta West District of the Western Region of Ghana. Swabs from lymphedematous limb ulcers, pus, and cutaneous surfaces were cultured using standard culture-based techniques. The culture isolates were subjected to Matrix-Assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) mass spectrometry for bacterial identification. Antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer method. mecA genes were targeted by polymerase chain reaction for strains that were cefoxitin resistant.

Results

In all, 112 S. hominis were isolated. The AST results showed resistance to chloramphenicol (87.5%), tetracycline (83.3%), penicillin (79.2%), and trimethoprim/sulphamethoxazole (45.8%). Of the 112 strains of S. hominis, 51 (45.5%) were resistant to cefoxitin, and 37 (72.5%) of the cefoxitin-resistant S. hominis haboured the mecA gene.

Conclusion

This study indicates a heightened level of methicillin-resistant S. hominis isolated among filarial lymphedema patients. As a result, opportunistic infections of S. hominis among the already burdened filarial lymphedema patients in rural Ghana may have reduced treatment success with antibiotics.

SUBMITTER: Kini P

PROVIDER: S-EPMC9904197 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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Antibiotic resistance and <i>mec</i>A characterization of <i>Staphylococcus hominis</i> from filarial lymphedema patients in the Ahanta West District, Ghana: A cross-sectional study.

Kini Priscilla P Wireko Solomon S Osei-Poku Priscilla P Asiedu Samuel O SO Amewu Emmanuel K A EKA Asiedu Ebenezer E Amanor Ernest E Mensah Caleb C Wilson Mary B MB Larbi Amma A Boahen Kennedy G KG Sylverken Augustina A AA Amato Katherine R KR Kwarteng Alexander A

Health science reports 20230207 2

<h4>Background and aim</h4>Filarial infections affect over 150 million people in the tropics. One of the major forms of filarial pathologies is lymphedema; a condition where the immune response is significantly altered, resulting in changes in the normal flora. <i>Staphylococcus hominis</i>, a human skin commensal, can also be pathogenic in immunocompromised individuals. Therefore, there is the possibility that <i>S. hominis</i> could assume a different behavior in filarial lymphedema patients. ...[more]

PMID: 36778776

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