Impaired calcium handling mechanisms in atrial trabeculae of diabetic patients.

ABSTRACT: The aim of this study was to investigate cardiomyocyte Ca²⁺ handling and contractile function in freshly excised human atrial tissue from diabetic and non-diabetic patients undergoing routine surgery. Multicellular trabeculae (283 ± 20 μm in diameter) were dissected from the endocardial surface of freshly obtained right atrial appendage samples from consenting surgical patients. Trabeculae were mounted in a force transducer at optimal length, electrically stimulated to contract, and loaded with fura-2/AM for intracellular Ca²⁺ measurements. The response to stimulation frequencies encompassing the physiological range was recorded at 37°C. Myofilament Ca²⁺ sensitivity was assessed from phase plots and high potassium contractures of force against [Ca²⁺

SUBMITTER: Jones TLM 

PROVIDER: S-EPMC9904963 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature