Flavescence doree phytoplasma enters insect cells by a clathrin-mediated endocytosis allowing infection of its insect vector.

ABSTRACT: To perform its propagative and circulative cycle into its insect vector, the flavescence dorée phytoplasma invades different cell types. Clathrin-mediated endocytosis is used by a wide range of bacteria to infect eukaryote cells. Among the insect proteins interacting with the phytoplasma adhesin VmpA, we identified the adaptor protein complex AP-1 and AP-2 suggesting that phytoplasmas could enter the insect cells via clathrin-mediated endocytosis. By infection assays of insect cells in culture, we showed that phytoplasmas entry into Drosophila S2 cells was more efficient than infection of the Euva cell line developed from the insect vector Euscelidius variegatus. Chlorpromazine, cytochalasin D and knockdown of clathrin heavy chain (chc) gene expression using RNA interference inhibited entr

SUBMITTER: Arricau-Bouvery N 

PROVIDER: S-EPMC9905606 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature