Expansion microscopy reveals subdomains in <i>C. elegans</i> germ granules.

ABSTRACT: Light and electron microscopy techniques have been indispensable in the identification and characterization of liquid-liquid phase separation membraneless organelles. However, for complex membraneless organelles such as the perinuclear germ granule in C. elegans, our understanding of how the intact organelle is regulated is hampered by (1) technical limitations in confocal fluorescence imaging for the simultaneous examination of multiple granule protein markers and (2) inaccessibility of electron microscopy. We take advantage of the newly developed super resolution method of expansion microscopy (ExM) and in situ staining of the whole proteome to examine the C. elegans germ granule, the P granule. We show that in small RNA pathway mutants, the P granule is smaller compared wi

SUBMITTER: Suen KM 

PROVIDER: S-EPMC9905708 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature