Effects and mechanisms of Celastrol on the formation of neutrophil extracellular traps (NETs).

ABSTRACT:

Background

To investigate the effect and mechanism of Celastrol on the formation of neutrophil extracellular traps (NETs), and to provide a theoretical basis for the clinical application of Tripterygium wilfordii.

Methods

First, we isolated neutrophils from the peripheral blood of healthy volunteers, and then observed the effect of Celastrol on Phorbol Myristate Acetate (PMA)-induced neutrophil release of NETs. The level of NETs was detected by using the membrane-impermeable nucleic acid dye, SytoxGreens. In addition, the levels of reactive oxygen species (ROS) were also examined to determine whether Celastrol affects ROS production during PMA-induced NETs.

Results

Celastrol produced significant cytotoxicity at a concentration of 5 µM (213.2±75.07), and the effect of

SUBMITTER: Song R 

PROVIDER: S-EPMC9906213 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature