A bioorthogonal chemistry approach to detect the K1 polysialic acid capsule in Escherichia coli.

ABSTRACT: Most Escherichia coli strains associated with neonatal meningitis express the K1 capsule, a sialic acid polysaccharide that is directly related to their pathogenicity. Metabolic oligosaccharide engineering (MOE) has mostly been developed in eukaryotes, but has also been successfully applied to the study of several oligosaccharides or polysaccharides constitutive of the bacterial cell wall. However, bacterial capsules are seldom targeted despite their important role as virulence factors, and the K1 polysialic acid (PSA) antigen that shields bacteria from the immune system still remains untackled. Herein, we report a fluorescence microplate assay that allows the fast and facile detection of K1 capsules with an approach that combines MOE and bioorthogonal chemistry. We exploit the inco

SUBMITTER: Rigolot V 

PROVIDER: S-EPMC9906323 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature