Deciphering chemical logic of fungal natural product biosynthesis through heterologous expression and genome mining.

ABSTRACT: Covering: 2010 to 2022Heterologous expression of natural product biosynthetic gene clusters (BGCs) has become a widely used tool for genome mining of cryptic pathways, bottom-up investigation of biosynthetic enzymes, and engineered biosynthesis of new natural product variants. In the field of fungal natural products, heterologous expression of a complete pathway was first demonstrated in the biosynthesis of tenellin in Aspergillus oryzae in 2010. Since then, advances in genome sequencing, DNA synthesis, synthetic biology, etc. have led to mining, assignment, and characterization of many fungal BGCs using various heterologous hosts. In this review, we will highlight key examples in the last decade in integrating heterologous expression into genome mining and biosynthetic inves

SUBMITTER: Chiang CY 

PROVIDER: S-EPMC9906657 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature