A genetically encoded fluorescent sensor for manganese(II), engineered from lanmodulin.

ABSTRACT: The design of selective metal-binding sites is a challenge in both small-molecule and macromolecular chemistry. Selective recognition of manganese (II)-the first-row transition metal ion that tends to bind with the lowest affinity to ligands, as described by the Irving-Williams series-is particularly difficult. As a result, there is a dearth of chemical biology tools with which to study manganese physiology in live cells, which would advance understanding of photosynthesis, host-pathogen interactions, and neurobiology. Here we report the rational re-engineering of the lanthanide-binding protein, lanmodulin, into genetically encoded fluorescent sensors for Mn^II, MnLaMP1 and MnLaMP2. These sensors with effective K_d(Mn^II) of 29 and 7 µM, respectively, d

SUBMITTER: Park J 

PROVIDER: S-EPMC9907080 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature