Correlated gene modules uncovered by high-precision single-cell transcriptomics.

ABSTRACT: Correlations in gene expression are used to infer functional and regulatory relationships between genes. However, correlations are often calculated across different cell types or perturbations, causing genes with unrelated functions to be correlated. Here, we demonstrate that correlated modules can be better captured by measuring correlations of steady-state gene expression fluctuations in single cells. We report a high-precision single-cell RNA-seq method called MALBAC-DT to measure the correlation between any pair of genes in a homogenous cell population. Using this method, we were able to identify numerous cell-type specific and functionally enriched correlated gene modules. We confirmed through knockdown that a module enriched for p53 signaling predicted p53 regulatory targets more acc

SUBMITTER: Chapman AR 

PROVIDER: S-EPMC9907105 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature