Minimally invasive skin sampling and transcriptome analysis using microneedles for skin type biomarker research.

ABSTRACT:

Background

Minimally invasive skin sampling is used in various fields. In this study, we examined whether it was possible to obtain skin specimens using biocompatible microneedles composed of sodium hyaluronate and performed transcriptome analysis.

Materials and methods

Thirty-three subjects with different skin conditions, such as skin aging, skin hydration, skin pigmentation, oily skin and sensitive skin, were recruited. Skin types were evaluated based on age, non-invasive measurement devices, 10% lactic acid stinging test and visual assessment; the skin specimens were sampled from the face using microneedles. Total RNA was extracted, and microarray was performed. Correlations between various biomarkers and skin condition parameters were analysed.

Results

Several ski

SUBMITTER: Kim SH 

PROVIDER: S-EPMC9907599 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature