Evaluation of intercellular lipid lamellae in the stratum corneum by polarized microscopy.

ABSTRACT:

Background

Intercellular lipids contain a lamellar structure that glows in polarized images. It could be expected that the intercellular lipid content be estimated from the luminance values calculated from polarized images of stratum corneum strips. Therefore, we attempted to develop a method for simple and rapid evaluation of the intercellular lipid content through a procedure. Herein, we demonstrated a relationship between the luminance value and the amount of ceramides, one of the main components of intercellular lipids.

Materials and methods

The stratum corneum was collected from the forearm using slides with a pure rubber-based adhesive, which did not produce unnecessary luminescence under polarizing conditions. Images were analyzed using luminance indices. The positive

SUBMITTER: Asada N 

PROVIDER: S-EPMC9907717 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature